Identification of Schistosoma mansoni antigens recognized by spleen cells of C57B1/6 mice immunized with ultraviolet-irradiated cercariae

Int J Parasitol. 1994 Nov;24(7):943-50. doi: 10.1016/0020-7519(94)90158-9.

Abstract

Spleen cells of C57B1/6 mice immunized twice with Schistosoma mansoni cercariae attenuated by ultraviolet irradiation proliferated and produced interleukin-(II)-2 and/or II-4 in response to both soluble schistosomular and adult worm antigens of 72-68, 60-62, 50, 45, 29.5 and 28 kDa. All of these bands, except the 45 kDa, were also recognized by serum antibodies in Western blotting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Helminth / immunology*
  • B-Lymphocytes / immunology
  • Female
  • Interleukin-2 / biosynthesis
  • Interleukin-4 / biosynthesis
  • Larva / immunology
  • Larva / radiation effects
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Protozoan Vaccines
  • Schistosoma mansoni / immunology
  • Schistosoma mansoni / radiation effects
  • Schistosomiasis mansoni / immunology
  • Schistosomiasis mansoni / prevention & control*
  • Spleen / cytology
  • Spleen / immunology*
  • T-Lymphocytes / immunology
  • Ultraviolet Rays
  • Vaccination*
  • Vaccines, Attenuated / immunology*
  • Vaccines, Inactivated / immunology

Substances

  • Antigens, Helminth
  • Interleukin-2
  • Protozoan Vaccines
  • Vaccines, Attenuated
  • Vaccines, Inactivated
  • Interleukin-4