PCR amplification of variable sequence upstream of katG gene to subdivide strains of Mycobacterium tuberculosis complex

M Goyal; D Young; Y Zhang; P A Jenkins; R J Shaw

doi:10.1128/jcm.32.12.3070-3071.1994

PCR amplification of variable sequence upstream of katG gene to subdivide strains of Mycobacterium tuberculosis complex

J Clin Microbiol. 1994 Dec;32(12):3070-1. doi: 10.1128/jcm.32.12.3070-3071.1994.

Authors

M Goyal¹, D Young, Y Zhang, P A Jenkins, R J Shaw

Affiliation

¹ Department of Respiratory Medicine, St. Mary's Hospital Medical School, London, United Kingdom.

Abstract

PCR amplification of a species-specific 2-kb KpnI fragment of variable size located 10 kb upstream of the katG gene was used to subdivide 130 clinical isolates of Mycobacterium tuberculosis. Seven subtypes were identified, and their frequencies were distributed normally with respect to the size of the amplified product.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques
DNA, Bacterial / analysis
Genes, Bacterial / genetics*
Genetic Variation
Humans
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics*
Polymerase Chain Reaction

Substances

DNA, Bacterial