RNA cleavage by C-1027 chromophore, an enediyne antitumor antibiotic: high selectivity to an anticodon arm

Biochem Biophys Res Commun. 1995 Mar 8;208(1):168-73. doi: 10.1006/bbrc.1995.1319.

Abstract

This study demonstrates unique reactivity of the C-1027 chromophore toward a tRNA(phe). In the presence of Mg2+ ions where the tRNA(phe) attains a stable three-dimensional structure, the enediyne chromophore exhibits high cleavage selectivity to the anticodon arm. The present reactivity of the C-1027 chromophore is useful in development of new chemical probes for mapping of tertiary RNA structure. Considering the paucity of RNA repair mechanisms, RNA may be also an important biological target for certain enediyne antibiotics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides*
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology*
  • Antibiotics, Antineoplastic / pharmacology*
  • Base Sequence
  • Enediynes
  • Molecular Sequence Data
  • Molecular Structure
  • Nucleic Acid Conformation*
  • Peptides*
  • RNA, Transfer, Phe / chemistry
  • RNA, Transfer, Phe / drug effects*
  • Saccharomyces cerevisiae
  • Spectrometry, Fluorescence

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • Antibiotics, Antineoplastic
  • Enediynes
  • Peptides
  • RNA, Transfer, Phe
  • C 1027