Abstract
Interleukin 3 is a cytokine that stimulates proliferation and differentiation of hematopoietic progenitor cells. Its receptor consists of two subunits, an interleukin 3-specific alpha subunit and a beta subunit shared by garanulocyte-macrophage colony stimulating factor and interleukin 5 receptors. In this paper, we determined the genomic structure of the alpha subunit of the human interleukin 3 receptor, which spans approximately 40 kb and has 12 exons. We found that the genomic structures of the alpha subunits of the human interleukin 3 and granulocyte-macrophage colony stimulating factor receptors are very similar. They possess a unique additional intron in the 'C domain', which is absent in the alpha subunit of the interleukin 5 receptor. These results suggest a shared evolutionary pathway of these two genes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Biological Evolution
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Blotting, Southern
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Cloning, Molecular
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Conserved Sequence
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DNA Primers
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Exons
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Humans
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Introns
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Macromolecular Substances
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Molecular Sequence Data
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Polymerase Chain Reaction
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Receptors, Cytokine / genetics*
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / genetics
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Receptors, Interleukin / genetics
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Receptors, Interleukin-3 / biosynthesis
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Receptors, Interleukin-3 / chemistry
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Receptors, Interleukin-3 / genetics*
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Receptors, Interleukin-5
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Restriction Mapping
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Sequence Homology, Nucleic Acid
Substances
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DNA Primers
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Macromolecular Substances
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Receptors, Cytokine
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
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Receptors, Interleukin
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Receptors, Interleukin-3
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Receptors, Interleukin-5
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Recombinant Proteins