Cloning and expression of the cDNA encoding rabbit liver carbonyl reductase

Gene. 1995 Mar 10;154(2):297-8. doi: 10.1016/0378-1119(94)00843-h.

Abstract

Two cDNA sequences encoding rabbit carbonyl reductase (CBR) were cloned from a lambda gt10 rabbit liver cDNA library. The rabbit cDNAs coded for a protein with 84% identity to human CBR. Transient expression of the two rabbit cDNA sequences in COS7 cells increased both quinone reductase and aldo-keto reductase activities. These data demonstrate that CBR cDNAs from rabbit and human tissues code for similar proteins.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcohol Oxidoreductases / biosynthesis*
  • Alcohol Oxidoreductases / chemistry
  • Alcohol Oxidoreductases / genetics*
  • Aldehyde Reductase
  • Aldo-Keto Reductases
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Chlorocebus aethiops
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Genes
  • Humans
  • Liver / enzymology*
  • Molecular Sequence Data
  • NAD(P)H Dehydrogenase (Quinone) / biosynthesis
  • RNA, Messenger / analysis
  • Rabbits
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Alcohol Oxidoreductases
  • Aldo-Keto Reductases
  • Aldehyde Reductase
  • NAD(P)H Dehydrogenase (Quinone)

Associated data

  • GENBANK/U07051
  • GENBANK/U09244