Abstract
Corticotropin synthesis and secretion is under negative feedback regulation by glucocorticoids. In addition a hypothalamic factor inhibiting ACTH synthesis and secretion, named corticotropin release inhibiting factor (CRIF), has been postulated but not identified. Here we report that transient transfection of a rat prepro-TRH cDNA into the mouse anterior pituitary tumor cell line AtT-20 inhibits the synthesis and secretion of both basal and CRH-stimulated ACTH. Thus, CRIF appears to be encoded by the same precursor as TRH, suggesting a coordinated regulation of pituitary-adrenal and pituitary-thyroid functions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenocorticotropic Hormone / antagonists & inhibitors*
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Adrenocorticotropic Hormone / biosynthesis
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Adrenocorticotropic Hormone / metabolism
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Animals
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Corticotropin-Releasing Hormone / pharmacology
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DNA, Complementary / genetics
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Mice
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Pituitary Gland, Anterior / metabolism*
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Pituitary Neoplasms
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Protein Precursors / genetics*
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Thyrotropin-Releasing Hormone / genetics*
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Transfection
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Tumor Cells, Cultured
Substances
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DNA, Complementary
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Protein Precursors
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prepro-thyrotropin-releasing hormone
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Thyrotropin-Releasing Hormone
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Adrenocorticotropic Hormone
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Corticotropin-Releasing Hormone