Streptomyces antibioticus strain TU 99, from which a wide variety of active compounds had been isolated previously, was reinvestigated using an HPLC photoconductivity screening system. Four new compounds were isolated, characterized and their constitutions determined. All four were alpha, beta-unsaturated gamma-lactones; the most abundant compound 3 (C10H16O4), as well as compound 1 (C9H14O4) had a hydroxy group at C(5) of the lactone ring. The four lactones showed antibiotic activity against Pseudomonas aeruginosa and also a weak inhibition of the chitinase from Serratia marcescens.