Application of fluorescence in situ hybridization in defining a complex t(9;21;22) Ph formation

Haematologica. 1994 Nov-Dec;79(6):536-9.

Abstract

We describe the application of fluorescence in situ hybridization (FISH) in a case of suspected chronic myelogenous leukemia (CML), cytogenetically characterized by a t(21;22) with no clear involvement of chromosome 9. The dual color FISH technique, performed using specific painting probes for chromosomes 9,21,22 and a BCR/ABL translocation probe, enabled us to confirm the diagnosis of CML by detecting the BCR/ABL rearrangement on chromosome 22q and the involvement of chromosome 9 in a variant translocation t(9;21;22).

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Chromosomes, Human, Pair 21 / ultrastructure*
  • Chromosomes, Human, Pair 22 / ultrastructure*
  • Chromosomes, Human, Pair 9 / ultrastructure*
  • Fusion Proteins, bcr-abl / genetics
  • Humans
  • In Situ Hybridization, Fluorescence*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology
  • Male
  • Philadelphia Chromosome*
  • Translocation, Genetic*

Substances

  • Fusion Proteins, bcr-abl