Abstract
Tomato (Lycopersicon esculentum L. cv. UC82b) was found to contain two distinct 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase genes that are differentially expressed. The corresponding cDNAs were isolated and characterized. Both genes code for putative plastidic DAHP synthase isoforms. The deduced amino acid sequences are 79% identical. A comparison of the known Solanaceae DAHP synthases indicates two distinct conserved isoforms. The steady-state levels of transcripts of the two tomato genes differ in all organs analysed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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3-Deoxy-7-Phosphoheptulonate Synthase / genetics*
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Amino Acid Sequence
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Arabidopsis / genetics
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Base Sequence
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Cell Compartmentation
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Cloning, Molecular
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Cytosol / enzymology
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DNA Primers / chemistry
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DNA, Complementary / genetics
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Gene Expression Regulation*
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Gene Library
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Genes, Plant*
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Isoenzymes / genetics
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Molecular Sequence Data
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Plants / anatomy & histology
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Plants / genetics*
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Plastids / enzymology
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Poly A / metabolism
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RNA Processing, Post-Transcriptional
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RNA, Messenger / genetics
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Sequence Alignment
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Sequence Homology, Amino Acid
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Shikimic Acid / metabolism
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Solanum tuberosum / genetics
Substances
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DNA Primers
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DNA, Complementary
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Isoenzymes
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RNA, Messenger
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Poly A
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Shikimic Acid
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3-Deoxy-7-Phosphoheptulonate Synthase
Associated data
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GENBANK/Z21792
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GENBANK/Z21793