Nosocomial spread of Staphylococcus aureus showing intermediate resistance to methicillin

J Hosp Infect. 1993 Oct;25(2):91-6. doi: 10.1016/0195-6701(93)90099-l.

Abstract

A nosocomial outbreak of infection and colonization involving six patients and caused by a strain of Staphylococcus aureus showing intermediate resistance to methicillin (MIC = 4-8 mg l-1) is described. The outbreak was associated with skin-carriage of the epidemic strain by a nurse suffering from severe eczema. The reduced susceptibility of the outbreak strain to methicillin was associated with beta-lactamase production. Elimination or inhibition of beta-lactamase activity produced a two-fold decrease in methicillin MIC. There was no evidence for the presence of either penicillin-binding protein 2a or the corresponding mec gene, which mediate resistance in fully methicillin-resistant strains.

MeSH terms

  • Carrier State / microbiology
  • Colony Count, Microbial
  • Cross Infection / epidemiology*
  • Cross Infection / microbiology
  • Cross Infection / transmission
  • Disease Outbreaks*
  • Female
  • Humans
  • Male
  • Methicillin Resistance*
  • Microbial Sensitivity Tests
  • Middle Aged
  • Nursing Staff, Hospital
  • Staphylococcal Infections / epidemiology*
  • Staphylococcal Infections / microbiology
  • Staphylococcal Infections / transmission
  • Staphylococcus aureus / enzymology
  • Staphylococcus aureus / isolation & purification*
  • United Kingdom / epidemiology
  • beta-Lactamases / biosynthesis

Substances

  • beta-Lactamases