Abstract
Point mutations of the Syrian hamster neu proto-oncogene have been observed in the transmembrane domain of N-nitroso-N-ethylurea (ENU)-induced neurofibromas. Genomic DNA derived from tumor tissue showed transforming activity in an NIH 3T3 assay and a cDNA clone of the hamster neu gene, containing the entire protein-coding region, was isolated from a transformant cDNA library. The encoded product is 92 and 88% homologous to the rat neu and the human c-erbB-2, respectively. The product of the mutated hamster neu gene showed increased autophosphorylation of Tyr residues.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Cricetinae
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ErbB Receptors / genetics*
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Gene Expression Regulation*
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Humans
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Mesocricetus
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Mice
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Molecular Sequence Data
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Phosphorylation
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogenes*
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Rats
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Receptor, ErbB-2
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Transfection
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Transformation, Genetic
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Tyrosine / metabolism
Substances
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MAS1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Tyrosine
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ErbB Receptors
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Receptor, ErbB-2