Abstract
Monocyte chemotactic proteins (MCPs) are chemokines involved in macrophage recruitment during inflammation and cancer. A full-size MCP-3 cDNA was used to isolate the functional human MCP-3 gene. Based on restriction analysis, subclones were selected and the MCP-3 gene sequence was completed. In addition to a dense region with direct and inverted repeats and palindromic sequences, a double microsatellite (CA)n-(GA)n' was found at the 5'-end of the MCP-3 gene, and an RFLP was detected. The gene was regionally mapped by fluorescence in situ hybridization to human chromosome 17, subbands q11.2-q12. This site contains the MCP-subset of C-C chemokines and can be distinguished from the syntenic MIP-1 alpha locus. SCYA7 was assigned as the locus symbol of the MCP-3 gene. Double-labeling experiments confirmed the regional assignment of the MCP-3 gene close to the ERBB2 locus on human chromosome 17.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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Chemokine CCL7
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Chemotactic Factors / genetics*
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Chromosome Mapping
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Chromosomes, Human, Pair 17*
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Cloning, Molecular
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Consensus Sequence
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Cytokines / genetics*
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DNA, Satellite / genetics
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Deoxyribonuclease BamHI
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ErbB Receptors / genetics
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Female
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Hominidae / genetics*
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Humans
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In Situ Hybridization, Fluorescence
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Introns
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Male
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Molecular Sequence Data
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Monocyte Chemoattractant Proteins*
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Multigene Family*
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Pedigree
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Polymorphism, Restriction Fragment Length*
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Proto-Oncogene Proteins / genetics
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Receptor, ErbB-2
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Regulatory Sequences, Nucleic Acid
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Repetitive Sequences, Nucleic Acid*
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Restriction Mapping
Substances
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CCL7 protein, human
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Chemokine CCL7
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Chemotactic Factors
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Cytokines
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DNA, Satellite
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Monocyte Chemoattractant Proteins
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Proto-Oncogene Proteins
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ErbB Receptors
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Receptor, ErbB-2
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Deoxyribonuclease BamHI
Associated data
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GENBANK/X72304
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GENBANK/X72308
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GENBANK/X72309