Isolation and sequence determination of cDNA encoding human T-protein of the glycine cleavage system

Biochem Biophys Res Commun. 1993 Apr 30;192(2):766-71. doi: 10.1006/bbrc.1993.1480.

Abstract

We present the full-length cDNA sequence of human T-protein of the glycine cleavage system. Overlapping cDNA clones were isolated by screening human liver cDNA libraries with bovine T-protein cDNA. The 1209 base pair open reading frame encodes 403 amino acid precursor protein, and the deduced amino acid sequence of the mature peptide shows 90 and 68% homology to that of bovine and chicken counterpart, respectively. RNA blot analysis of human liver transcripts and Southern blot analysis of human genomic DNA confirm that human T-protein is encoded by a single gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Aminomethyltransferase
  • Animals
  • Base Sequence
  • DNA
  • Humans
  • Hydroxymethyl and Formyl Transferases*
  • Liver / embryology
  • Liver / enzymology
  • Molecular Sequence Data
  • Open Reading Frames
  • Sequence Homology, Amino Acid
  • Transferases / genetics*

Substances

  • DNA
  • Transferases
  • Hydroxymethyl and Formyl Transferases
  • Aminomethyltransferase

Associated data

  • GENBANK/D13811
  • GENBANK/D14597
  • GENBANK/D14598
  • GENBANK/D14599
  • GENBANK/D14600
  • GENBANK/D14601
  • GENBANK/D14602
  • GENBANK/D14603
  • GENBANK/S64636
  • GENBANK/S64637