Abstract
The gene for human uracil-DNA glycosylase (UNG) contains 4 exons and has an approximate size of 13 kb. The promoter is very GC rich and lacks a TATA box. Nested deletions of the promoter demonstrated that two SP1 elements and a putative c-MYC element proximal to the transcription initiation region were sufficient to support some 27% of the promoter activity, while a clone that in addition contained the elements E2F/SP1/CCAAT increased expression to almost 90% of the full-length construct. A region upstream of these elements appears to exert a negative control function.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Binding Sites
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Blotting, Southern
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DNA / chemistry*
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DNA Glycosylases*
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DNA Restriction Enzymes
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Exons
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Humans
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Molecular Sequence Data
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N-Glycosyl Hydrolases / genetics*
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Promoter Regions, Genetic*
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Proto-Oncogene Proteins c-myc / metabolism
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Restriction Mapping
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Sp1 Transcription Factor / metabolism
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TATA Box
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Transcription Factors / metabolism
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Uracil-DNA Glycosidase
Substances
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Proto-Oncogene Proteins c-myc
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Sp1 Transcription Factor
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Transcription Factors
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DNA
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DNA Restriction Enzymes
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DNA Glycosylases
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N-Glycosyl Hydrolases
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Uracil-DNA Glycosidase