Expression of monocyte chemoattractant protein 1 in human osteoblastic cells stimulated by proinflammatory mediators

J Bone Miner Res. 1994 Jul;9(7):1123-30. doi: 10.1002/jbmr.5650090721.

Abstract

Monocyte chemoattractant protein 1 (MCP-1) is a member of the chemokine superfamily of genes that induces chemotaxis of monocytes in inflammatory processes. The effects of interleukin-1 beta (IL-1 beta), tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), transforming growth factor beta (TGF-beta), platelet-derived growth factor (PDGF-BB), parathyroid hormone (PTH), and 1,25(OH)2D3 on MCP-1 expression in human osteoblastic cells were compared. Inflammatory or proinflammatory cytokines stimulated the production of MCP-1 in normal human osteoblastic cells as determined by RIA. The osteotrophic mediators PTH and 1,25(OH)2D3 and PDGF-BB had no effect on MCP-1 expression. In further studies, the steady-state mRNA and MCP-1 protein levels in two human osteoblastic cell lines, MG-63 and SaOS-2, were examined. MCP-1 expression at both the protein and mRNA levels was greatly increased by IL-1 beta and TNF-alpha. At the mRNA level, IL-1 beta and TNF-alpha strongly induced MCP-1 expression; TGF-beta and IL-6 induced MCP-1 but to a lesser extent. No significant changes in MCP-1 mRNA or MCP-1 protein secretion were observed when cells were treated with PDGF-BB, PTH, and 1,25(OH)2D3. When tested on preosteoclasts, MCP-1 was shown to have no effect on the formation of multinucleated, tartrate-resistant acid phosphatase (TRAP)-positive osteoclastic cells.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Autoradiography
  • Becaplermin
  • Blotting, Northern
  • Calcitriol / pharmacology
  • Cells, Cultured
  • Chemokine CCL2
  • Chemotactic Factors / biosynthesis
  • Chemotactic Factors / genetics*
  • Cytokines / pharmacology*
  • Humans
  • Interleukin-1 / pharmacology
  • Interleukin-6 / pharmacology
  • Osteoblasts / drug effects
  • Osteoblasts / metabolism*
  • Parathyroid Hormone / pharmacology
  • Platelet-Derived Growth Factor / pharmacology
  • Proto-Oncogene Proteins c-sis
  • Radioimmunoassay
  • Recombinant Proteins / pharmacology
  • Transforming Growth Factor beta / pharmacology
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Chemokine CCL2
  • Chemotactic Factors
  • Cytokines
  • Interleukin-1
  • Interleukin-6
  • Parathyroid Hormone
  • Platelet-Derived Growth Factor
  • Proto-Oncogene Proteins c-sis
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • Tumor Necrosis Factor-alpha
  • Becaplermin
  • Calcitriol