Different types of structural variation in STRs: HumFES/FPS, HumVWA and HumD21S11

Int J Legal Med. 1994;106(6):319-23. doi: 10.1007/BF01224779.

Abstract

Alleles of the STR systems HumFES/FPS, HumVWA and HumD21S11 were sequenced and analyzed. Sequence data revealed 3 different systems concerning the complexity of their sequence structure. HumFES/FPS belongs to the STR polymorphism with a simple repeat structure. Only 2 subtypes were found with a base substitution in the 5'-flanking region and no variation in the repeat region. In the STR system HumVWA the sequence structure of the repeat region is more complex, because 2 tetranucleotide units TCTA and TCTG were present. Additionally allele 14 revealed a completely different sequence structure leading to a different electrophoretic mobility. The repeat region of HumD21S11 is compound in structure. The possibility of variation at 3 positions leads to the occurrence of microheterogeneities in fragments of apparent length. In the upper allele range alleles arise with an additional incomplete TA-repeat.

MeSH terms

  • Alleles
  • Base Sequence
  • DNA*
  • Electrophoresis, Polyacrylamide Gel
  • Gene Frequency
  • Genetic Heterogeneity
  • Humans
  • Molecular Sequence Data
  • Polymorphism, Restriction Fragment Length*
  • Repetitive Sequences, Nucleic Acid* / genetics
  • Sequence Analysis, DNA*
  • White People / genetics

Substances

  • DNA