Modulation of LDL catabolism by sodium nitroprusside and PGE2 in human peripheral blood lymphocytes

B Miszczuk-Jamska; I Wybrańska; J Hartwich; A Dembińska-Kieć; R J Gryglewski

Modulation of LDL catabolism by sodium nitroprusside and PGE2 in human peripheral blood lymphocytes

J Physiol Pharmacol. 1994 Jun;45(2):259-69.

Authors

B Miszczuk-Jamska¹, I Wybrańska, J Hartwich, A Dembińska-Kieć, R J Gryglewski

Affiliation

¹ Department of Clinical Biochemistry Collegium Medicum Jagiellonian University, Cracow, Poland.

PMID: 7949235

Abstract

The effect of nitric oxide donor--sodium nitroprusside (NaNP) and PGE2 on the LDL-receptor activity and LDL cellular accumulation by isolated human blood lymphocytes was investigated. Preincubation of lymphocytes with lipoprotein deficient medium (LPDS) resulted in the increase of the LDL-receptor activity and the LDL-cellular accumulation. NaNP (30-300 microM) dose-dependently prevented the increase of the LDL-receptor activity as well as the accumulation of LDL by lymphocytes. However, in "starwing" cells (cells with high LDL-receptor activity) the effect of NaNP on the receptor activity was biphasic. At concentration up to 100 microM NaNP inhibited, while at a concentration of 300 microM, it activated the LDL-receptor activity. PGE2 (3-30 microM) inhibited LDL catabolism, however, this effect was hardly concentration-dependent.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dinoprostone / pharmacology*
Humans
In Vitro Techniques
Iodine Radioisotopes
Lipoproteins, LDL / metabolism*
Lymphocytes / drug effects
Lymphocytes / metabolism*
Nitroprusside / pharmacology*
Ultracentrifugation
Up-Regulation / drug effects

Substances

Iodine Radioisotopes
Lipoproteins, LDL
Nitroprusside
Dinoprostone