X-ray crystallographic studies of eukaryotic transcription factors

Cold Spring Harb Symp Quant Biol. 1993:58:123-32. doi: 10.1101/sqb.1993.058.01.016.

Abstract

Our X-ray crystallographic work on eukaryotic transcription factors traverses the entire length of a typical class II nuclear gene promoter, including the TATA-box-binding protein, two b/HLH/Z promoter proximal binding factors (Max and USF), and an enhancer-binding factor (HNF-3 gamma). These high-resolution studies of specific protein/DNA interactions will be extended to include homologous proteins complexed with similar oligonucleotides and a systematic examination of the roles of individual amino acids and bases implicated in specific DNA binding. In the longer term, we will turn our attention to the myriad of protein/protein interactions occurring within TFIID and the PIC, and between the PIC and factors binding to promoter proximal and distal enhancer elements.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Basic-Leucine Zipper Transcription Factors
  • Binding Sites
  • Crystallography, X-Ray
  • DNA / chemistry
  • DNA / genetics
  • DNA / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Enhancer Elements, Genetic
  • Eukaryotic Cells
  • Hepatocyte Nuclear Factor 3-gamma
  • Histones / chemistry
  • Models, Molecular
  • Molecular Sequence Data
  • Molecular Structure
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Nucleic Acid Conformation
  • Promoter Regions, Genetic
  • Protein Conformation
  • RNA Polymerase II / metabolism
  • Transcription Factor TFIID
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Basic-Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • Histones
  • Myc associated factor X
  • Nuclear Proteins
  • Transcription Factor TFIID
  • Transcription Factors
  • Hepatocyte Nuclear Factor 3-gamma
  • DNA
  • RNA Polymerase II