Abstract
Our X-ray crystallographic work on eukaryotic transcription factors traverses the entire length of a typical class II nuclear gene promoter, including the TATA-box-binding protein, two b/HLH/Z promoter proximal binding factors (Max and USF), and an enhancer-binding factor (HNF-3 gamma). These high-resolution studies of specific protein/DNA interactions will be extended to include homologous proteins complexed with similar oligonucleotides and a systematic examination of the roles of individual amino acids and bases implicated in specific DNA binding. In the longer term, we will turn our attention to the myriad of protein/protein interactions occurring within TFIID and the PIC, and between the PIC and factors binding to promoter proximal and distal enhancer elements.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Base Sequence
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Basic-Leucine Zipper Transcription Factors
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Binding Sites
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Crystallography, X-Ray
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DNA / chemistry
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DNA / genetics
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DNA / metabolism
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Enhancer Elements, Genetic
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Eukaryotic Cells
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Hepatocyte Nuclear Factor 3-gamma
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Histones / chemistry
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Models, Molecular
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Molecular Sequence Data
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Molecular Structure
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Nucleic Acid Conformation
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Promoter Regions, Genetic
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Protein Conformation
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RNA Polymerase II / metabolism
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Transcription Factor TFIID
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Transcription Factors / chemistry*
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Basic-Leucine Zipper Transcription Factors
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DNA-Binding Proteins
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Histones
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Myc associated factor X
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Nuclear Proteins
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Transcription Factor TFIID
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Transcription Factors
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Hepatocyte Nuclear Factor 3-gamma
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DNA
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RNA Polymerase II