Abstract
The cloning of a 1332 bp cDNA from a potato (Solanum tuberosum L.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum. Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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DNA, Complementary*
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Escherichia coli / genetics
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Gene Dosage
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Gene Expression Regulation, Plant
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Gene Library
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Genes, Plant / genetics*
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Glycoside Hydrolases / genetics*
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Glycoside Hydrolases / metabolism
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Molecular Sequence Data
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Open Reading Frames / genetics
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Recombinant Fusion Proteins / biosynthesis
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Solanum tuberosum / enzymology
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Solanum tuberosum / genetics*
Substances
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DNA, Complementary
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Recombinant Fusion Proteins
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Glycoside Hydrolases