Recovery of duplications by drug resistance selection in Caenorhabditis elegans

Genome. 1994 Aug;37(4):701-5. doi: 10.1139/g94-098.

Abstract

We have devised a scheme that facilitates rapid screening for duplications of essential loci. Our scheme takes advantage of the lev-1(x22) mutation, which confers resistance in a recessive fashion to the potent anthelmintic levamisole. We have tested our methodology by recovering two gamma ray induced duplications of let-56, the first essential gene to the left of unc-22. One of the duplications is attached to the fourth chromosome. The other duplication is attached to the X chromosome. This duplication contains a functional copy of the unc-22 gene, as well as functional copies of several essential loci adjacent to unc-22. Results we have obtained during analysis of this duplication are compatible with the notion that the copy of the unc-22 gene located on the duplication is subject to X chromosome dosage compensation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / drug effects
  • Caenorhabditis elegans / genetics*
  • Chromosome Mapping
  • Crosses, Genetic
  • Dosage Compensation, Genetic
  • Drug Resistance / genetics
  • Female
  • Genes, Helminth*
  • Levamisole / pharmacology
  • Male
  • Multigene Family*
  • Mutagenesis
  • X Chromosome

Substances

  • Levamisole