Abstract
Genes encoding both the 66- and the 43-kDa subunits of yeast RNA polymerase II initiation factor a, designated TFA1 and TFA2, have been isolated. Both genes are essential for cell viability. The bacterially expressed gene products could replace factor a in transcription in vitro, and both recombinant subunits were required for activity. The deduced amino acid sequences of the TFA1 and TFA2 gene products were homologous to those of the large and small subunits of human TFIIE, respectively, identifying factor a as the yeast homolog of TFIIE.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Cell Survival
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DNA Primers / chemistry
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DNA, Fungal / genetics
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Fungal Proteins / genetics
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Gene Expression
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Genes, Fungal
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Humans
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Molecular Sequence Data
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RNA, Fungal / genetics
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RNA, Messenger / genetics
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Recombinant Proteins
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Saccharomyces cerevisiae
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / genetics*
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Transcription Factors, TFII*
Substances
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DNA Primers
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DNA, Fungal
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Fungal Proteins
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RNA, Fungal
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RNA, Messenger
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Recombinant Proteins
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Transcription Factors
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Transcription Factors, TFII
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transcription factor TFIIE
Associated data
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GENBANK/U12824
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GENBANK/U12825