Abstract
An ultrafiltration system was evaluated for the free-fraction measurement of SPECT radiotracers (beta-CIT, IBF, and iomazenil) used in functional brain imaging. The effect of temperature, storage, centrifugal force, tracer concentration, and percentage filtered demonstrated a relative error of < 9%. As a result of the minimal temperature effect, 25 degrees C was employed for all measurements. A comparison of the ultrafiltration system with equilibrium dialysis revealed < 5% difference for beta-CIT and iomazenil, but 16% for IBF. Additionally, the time and ease of operation considerably favored the ultrafiltration system. The precision quantitated by repetition was < 6% for between-run and within-run variability. In conclusion, ultrafiltration provided rapid results, demonstrated minor analytical errors, revealed generally good correlation with equilibrium dialysis, and allowed excellent precision.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adsorption
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Benzofurans / analysis*
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Benzofurans / blood
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Benzofurans / pharmacokinetics
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Blood Proteins / metabolism
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Cocaine / analogs & derivatives*
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Cocaine / analysis
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Cocaine / blood
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Cocaine / pharmacokinetics
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Dialysis
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Evaluation Studies as Topic
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Flumazenil / analogs & derivatives*
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Flumazenil / analysis
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Flumazenil / blood
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Flumazenil / pharmacokinetics
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Humans
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Iodine Radioisotopes
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Membranes, Artificial
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Protein Binding
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Pyrrolidines / analysis*
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Pyrrolidines / blood
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Pyrrolidines / pharmacokinetics
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Reproducibility of Results
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Temperature
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Tomography, Emission-Computed, Single-Photon / methods
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Ultrafiltration / methods
Substances
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Benzofurans
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Blood Proteins
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Iodine Radioisotopes
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Membranes, Artificial
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Pyrrolidines
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5-iodo-N-((1-ethyl-2-pyrrolidinyl)methyl)-2,3-dihydrobenzofurancarboxamide
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Flumazenil
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2beta-carbomethoxy-3beta-(4-iodophenyl)tropane
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iomazenil
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Cocaine