Immunization with whole inactivated vaccine protects from infection by SIV grown in human but not macaque cells

J Med Primatol. 1994 Feb-May;23(2-3):75-82. doi: 10.1111/j.1600-0684.1994.tb00105.x.

Abstract

Homologous SIVsm stocks were generated by passage of a macaque isolate of SIVsm (SIVsm/E660) in human CEM x 174 cells or macaque peripheral blood mononuclear cells. Macaques were immunized with whole inactivated SIV vaccine consisting of virus generated by transfection of CEM x 174 cells with the SIVsmH4 clone and were challenged with either cell-free stock. Only vaccinees challenged with virus generated in human cells were protected from infection. This confirms the species-specificity of whole inactivated vaccine-mediated protection.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Cell Line
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Macaca mulatta
  • SAIDS Vaccines / immunology*
  • Simian Acquired Immunodeficiency Syndrome / immunology*
  • Simian Acquired Immunodeficiency Syndrome / prevention & control
  • Simian Immunodeficiency Virus / growth & development
  • Simian Immunodeficiency Virus / immunology*
  • Time Factors
  • Viremia / immunology
  • Viremia / prevention & control

Substances

  • Antibodies, Viral
  • SAIDS Vaccines