Infectivity of titered doses of simian immunodeficiency virus clone E11S inoculated intravenously into rhesus macaques (Macaca mulatta)

R E Benveniste; S T Roodman; R W Hill; W B Knott; J L Ribas; M G Lewis; G A Eddy

doi:10.1111/j.1600-0684.1994.tb00106.x

Infectivity of titered doses of simian immunodeficiency virus clone E11S inoculated intravenously into rhesus macaques (Macaca mulatta)

J Med Primatol. 1994 Feb-May;23(2-3):83-8. doi: 10.1111/j.1600-0684.1994.tb00106.x.

Authors

R E Benveniste¹, S T Roodman, R W Hill, W B Knott, J L Ribas, M G Lewis, G A Eddy

Affiliation

¹ Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, MD 21702-1201.

PMID: 7966238
DOI: 10.1111/j.1600-0684.1994.tb00106.x

Abstract

The macaque infectious dose (MID) of a single-cell clone of simian immunodeficiency virus isolated from a pig-tailed macaque (SIV/Mne clone E11S) was determined in rhesus macaques (Macaca mulatta). Twenty-one macaques were inoculated with 10-fold dilutions of the virus stock (three or four animals per dose). The virologic and clinical status of these animals was monitored for 26 weeks. The 25% MID (MID25) occurred at a 10(5)-fold dilution of the viral stock.

MeSH terms

Animals
Antibodies, Viral / blood
Base Sequence
DNA Primers
Immunoblotting
Macaca mulatta / virology*
Macaca nemestrina
Molecular Sequence Data
Polymerase Chain Reaction / methods
Sensitivity and Specificity
Simian Acquired Immunodeficiency Syndrome / immunology
Simian Acquired Immunodeficiency Syndrome / physiopathology
Simian Immunodeficiency Virus / growth & development
Simian Immunodeficiency Virus / isolation & purification
Simian Immunodeficiency Virus / pathogenicity*

Substances

Antibodies, Viral
DNA Primers