Abstract
Influenza virus nucleoprotein (NP) is associated with the genome RNA, forming ribonucleoprotein cores. To identify the amino acid sequence involved in RNA binding, we performed Northwestern blot analysis with a set of N- and C-terminal deletion mutants of NP produced in Escherichia coli. The RNA binding region has been mapped between amino acid residues 91 and 188, a stretch of residues that contains a sequence that is not only highly conserved among NPs from A-, B-, and C-type influenza viruses but also similar to the RNA binding domain of a plant virus movement protein.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Nucleus / metabolism
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli
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Immunoblotting
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Influenza A virus / genetics
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Influenza A virus / metabolism*
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Molecular Sequence Data
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Nucleocapsid Proteins
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Nucleoproteins / biosynthesis
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Nucleoproteins / isolation & purification
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Nucleoproteins / metabolism*
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Orthomyxoviridae / genetics
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Orthomyxoviridae / metabolism
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Plasmids
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism*
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Restriction Mapping
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Sequence Deletion*
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Sequence Homology, Amino Acid
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Viral Core Proteins / biosynthesis
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Viral Core Proteins / isolation & purification
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Viral Core Proteins / metabolism*
Substances
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NP protein, Influenza A virus
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Nucleocapsid Proteins
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Nucleoproteins
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RNA-Binding Proteins
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Recombinant Proteins
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Viral Core Proteins