Apoptotic cell death of T-lymphocytes in experimental autoimmune neuritis of the Lewis rat

Neurosci Lett. 1994 Jul 18;176(1):75-9. doi: 10.1016/0304-3940(94)90875-3.

Abstract

The present study describes apoptosis of T-lymphocytes in the sciatic nerve in experimental autoimmune neuritis (EAN), a T-cell mediated disorder of the peripheral nervous system (PNS). Morphological signs characteristic for apoptotic cell death were found from day 4 onwards, peaking at day 7. Apoptosis of T-cells could be detected throughout the subsequent recovery period. In situ nick translation (ISNT) followed by immunocytochemical analysis confirmed the presence of DNA fragmentation in T-lymphocytes. We thus show for the first time that apoptosis is not a unique elimination mechanism of inflammatory T-cells in the central nervous system (CNS), but is operative in the PNS as well although at a lesser degree. Identifying the underlying mechanisms may provide a basis for the development of new therapeutic strategies in autoimmune disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis*
  • DNA / analysis
  • DNA Damage
  • Female
  • Neuritis, Autoimmune, Experimental / immunology*
  • Neuritis, Autoimmune, Experimental / pathology
  • Rats
  • Rats, Inbred Lew
  • Sciatic Nerve / immunology*
  • Sciatic Nerve / pathology
  • T-Lymphocytes / pathology*
  • Time Factors

Substances

  • DNA