Intrastriatal implants of polymer-encapsulated PC12 cells: effects on motor function in aged rats

Prog Neuropsychopharmacol Biol Psychiatry. 1994 Sep;18(5):935-46. doi: 10.1016/0278-5846(94)90109-0.

Abstract

1. The feasibility of ameliorating the motor deficits in aged rats was evaluated in animals receiving polymer-encapsulated PC12 cells. 2. Motor coordination and balance was evaluated in young (5-6 month) and aged (24-25 month) rats. Compared to the young animals, the aged animals fell more rapidly from a rotating rod and were unable to maintain their balance on a series of wooden beams of varying widths. 3. Following baseline testing, aged animals received either no implant, empty capsules or PC12 cell-loaded capsules implanted bilaterally into the striatum. 4. Three weeks following surgery, animals were re-tested and a significant improvement in balance on the rotorod and wooden beams was observed in those aged animals receiving PC12 cell-loaded capsules. No improvements or decrements in performance were observed in those animals receiving empty. Histological analysis revealed the presence of surviving tyrosine hydroxylase-positive PC12 cells randomly distributed within the capsules.

MeSH terms

  • Animals
  • Chelating Agents
  • Chitin / analogs & derivatives
  • Chitosan
  • Dopamine / metabolism
  • Immunohistochemistry
  • Male
  • Motor Activity / physiology
  • Neostriatum / anatomy & histology
  • Neostriatum / physiology*
  • PC12 Cells*
  • Postural Balance / physiology
  • Psychomotor Performance / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Tyrosine 3-Monooxygenase / metabolism

Substances

  • Chelating Agents
  • Chitin
  • Chitosan
  • Tyrosine 3-Monooxygenase
  • Dopamine