Leukemia inhibitory factor: association with intraamniotic infection

Am J Obstet Gynecol. 1994 Nov;171(5):1335-41. doi: 10.1016/0002-9378(94)90157-0.

Abstract

Objective: Our purpose was to investigate whether leukemia inhibitory factor is associated with intraamniotic infection.

Study design: A comparative clinical study of amniotic fluid leukemia inhibitory factor concentrations was performed.

Results: Leukemia inhibitory factor was undetectable (< 1 ng/ml) by radioreceptor assay during normal pregnancy at midtrimester and at term. Among women in labor those with intraamniotic infection had higher leukemia factor concentrations than those without infection before term (p < 0.001) and at term (p < 0.005). The leukemia inhibitory factor concentrations correlated with the amniotic fluid white blood cell counts (r = 0.47) (p < 0.001). In cultured human gestational tissue explants, leukemia inhibitory factor release was significantly enhanced by endotoxin, interleukin-1 alpha, and tumor necrosis factor-alpha however, leukemia inhibitory factor did not enhance the release of prostaglandin E2 by these tissues.

Conclusions: Amniotic fluid leukemia inhibitory factor concentrations were elevated during intraamniotic infection and gestational tissues released leukemia inhibitory factor in response to bacterial products and inflammatory mediators.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amnion*
  • Amniotic Fluid / metabolism*
  • Bacterial Infections / metabolism*
  • Culture Techniques
  • Cytokines / pharmacology
  • Dinoprostone / metabolism
  • Female
  • Growth Inhibitors / metabolism*
  • Humans
  • Interleukin-6*
  • Leukemia Inhibitory Factor
  • Lipopolysaccharides / pharmacology
  • Lymphokines / metabolism*
  • Osmolar Concentration
  • Pregnancy
  • Trophoblasts / cytology
  • Trophoblasts / metabolism
  • Tumor Cells, Cultured

Substances

  • Cytokines
  • Growth Inhibitors
  • Interleukin-6
  • LIF protein, human
  • Leukemia Inhibitory Factor
  • Lipopolysaccharides
  • Lymphokines
  • Dinoprostone