Concentration of autoantibodies to native 60-kd Ro/SS-A and denatured 52-kd Ro/SS-A in eluates from the heart of a child who died with congenital complete heart block

Arthritis Rheum. 1994 Nov;37(11):1698-703. doi: 10.1002/art.1780371120.

Abstract

Objective: To determine the serologic specificity of acid eluates from tissues of a child who died with congenital complete heart block (CCHB).

Methods: Tissues were extracted, acid eluted, and the IgG and antibody titers determined on the eluates by enzyme-linked immunosorbent assay.

Results: Antibodies to native 60-kd and denatured 52-kd Ro/SS-A were found to be enriched only in the heart eluate, and not in the eluates from brain, kidney, and skin.

Conclusion: These findings indicate a major role for anti-native 60-kd Ro/SS-A in the immunopathogenesis of CCHB.

Publication types

  • Case Reports
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Autoantibodies / analysis*
  • Autoantigens / immunology*
  • Autoantigens / metabolism
  • Child
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fetal Death / immunology*
  • Fluorescent Antibody Technique
  • Heart Block / congenital*
  • Heart Block / immunology*
  • Humans
  • Molecular Weight
  • Myocardium / immunology*
  • Pregnancy
  • Protein Denaturation
  • RNA, Small Cytoplasmic*
  • Ribonucleoproteins / immunology*
  • Ribonucleoproteins / metabolism

Substances

  • Autoantibodies
  • Autoantigens
  • RNA, Small Cytoplasmic
  • RO60 protein, human
  • Ribonucleoproteins
  • SS-A antigen