Abstract
Nef is a 27-kDa myristylated protein conserved in most human immunodeficiency virus (HIV)-1, HIV-2, and simian immunodeficiency virus isolates. Simian immunodeficiency virus Nef is required in macaques for both high viral load and full pathological effects. Nef down-regulates the cell surface expression of CD4 by a post-translational mechanism that is not yet fully elucidated. We have used the yeast two-hybrid system to identify cellular proteins that interact with Nef. A cDNA was isolated which encodes a COOH-terminal fragment of human beta-COP, a major coat component of non-clathrin-coated vesicles. Nef and beta-COP interacted in vitro and were found to be physically associated in HIV-1-infected cells by co-immunoprecipitation. These observations suggest that beta-COP might be one of the cellular mediators of Nef function in HIV-1-infected cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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Coated Pits, Cell-Membrane / metabolism*
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Coatomer Protein
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Conserved Sequence
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DNA Primers
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DNA, Complementary / isolation & purification
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Gene Products, nef / biosynthesis
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Gene Products, nef / chemistry
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Gene Products, nef / metabolism*
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HIV-1 / metabolism*
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HIV-2 / metabolism
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Humans
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Macaca
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Membrane Proteins / metabolism
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Microtubule-Associated Proteins / biosynthesis
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Microtubule-Associated Proteins / chemistry
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Microtubule-Associated Proteins / metabolism*
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Molecular Sequence Data
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Polymerase Chain Reaction
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Rats
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Sequence Homology, Amino Acid
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Simian Immunodeficiency Virus / metabolism
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nef Gene Products, Human Immunodeficiency Virus
Substances
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Coatomer Protein
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DNA Primers
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DNA, Complementary
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Gene Products, nef
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Membrane Proteins
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Microtubule-Associated Proteins
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nef Gene Products, Human Immunodeficiency Virus