Abstract
We examined the protective effects of idebenone, 6-(10-hydroxydecyl)-2,3-dimethoxy-5-methyl-1,4-benzoquinone, on the cytotoxicity of oxidatively-modified low density lipoprotein (oxLDL), using cultured vascular endothelial cells from fetal bovine aorta. When the cells were incubated with idebenone, the toxicity of oxLDL was inhibited dose-dependently (10(-7)-10(-5) M). When cells were preincubated with idebenone, the toxicity of oxLDL was inhibited only at a high concentration (10(-5) M). However, idebenone had no significant effects on copper-induced modification of LDL. The protective effects of idebenone on oxLDL-induced endothelial toxicity may be beneficial for the inhibition of the development of atherosclerosis in the brain and other arterial systems.
MeSH terms
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Animals
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Aorta, Thoracic
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Benzoquinones / pharmacology*
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Butylated Hydroxytoluene / pharmacology
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Cattle
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Cell Survival / drug effects
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Cells, Cultured
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Copper / pharmacology
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Dose-Response Relationship, Drug
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects*
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Humans
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Kinetics
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L-Lactate Dehydrogenase / analysis
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Lipid Peroxidation
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Lipoproteins, LDL / blood
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Lipoproteins, LDL / drug effects
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Lipoproteins, LDL / isolation & purification
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Malondialdehyde / analysis
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Thiobarbituric Acid Reactive Substances / analysis
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Ubiquinone / analogs & derivatives
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Vitamin E / pharmacology
Substances
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Benzoquinones
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Lipoproteins, LDL
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Thiobarbituric Acid Reactive Substances
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Ubiquinone
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Vitamin E
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Butylated Hydroxytoluene
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Malondialdehyde
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Copper
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L-Lactate Dehydrogenase
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idebenone