Abstract
A simplified method for large-scale HPA-1a phenotyping of platelets was developed for use in an antenatal screening programme for fetomaternal alloimmune thrombocytopenia (FMAIT). The test was based on the MAIPA assay, which was modified for antigen-typing with a well-characterized anti-HPA-1a reagent. The resulting assay gave reliable results, was inexpensive and allowed testing of large batches using semiautomated equipment.
MeSH terms
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Antibodies, Monoclonal / immunology
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Antigens, Human Platelet / analysis*
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Antigens, Human Platelet / genetics
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Databases, Factual
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Female
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Genetic Testing* / economics
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Humans
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Immunoenzyme Techniques
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Integrin beta3
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Phenotype
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Pregnancy / blood*
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Pregnancy / immunology
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Serology
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Thrombocytopenia / genetics
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Thrombocytopenia / immunology
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Thrombocytopenia / prevention & control*
Substances
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Antibodies, Monoclonal
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Antigens, Human Platelet
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ITGB3 protein, human
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Integrin beta3