Abstract
Nearly precise excision of a transposon related to Tn10 from an Escherichia coli plasmid was used as a model to study illegitimate DNA recombination between short direct repeats. The excision was stimulated 100-1000 times by induction of plasmid single-stranded DNA synthesis and did not involve transfer of DNA from the parental to the progeny molecule. We conclude that it occurred by copy-choice DNA recombination, and propose that other events of recombination between short direct repeats might be a result of the same process.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA Replication / genetics
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DNA Transposable Elements / genetics*
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DNA, Recombinant / metabolism
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DNA, Single-Stranded / biosynthesis
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Escherichia coli / genetics
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Escherichia coli / growth & development
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Inoviridae / physiology
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Models, Genetic
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Molecular Sequence Data
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Multigene Family / genetics*
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Nucleic Acid Conformation
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Plasmids / genetics
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Plasmids / metabolism*
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Recombination, Genetic / genetics*
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Repetitive Sequences, Nucleic Acid*
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Viral Proteins / genetics
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Viral Proteins / metabolism
Substances
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DNA Transposable Elements
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DNA, Recombinant
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DNA, Single-Stranded
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Viral Proteins