Partial purification of a putative membrane bound androgen binding protein in the dog cauda epididymis

J A Oduma; E N Waindi; S Gombe; E O Wango

doi:10.1016/0020-711x(94)90017-5

Partial purification of a putative membrane bound androgen binding protein in the dog cauda epididymis

Int J Biochem. 1994 Apr;26(4):583-8. doi: 10.1016/0020-711x(94)90017-5.

Authors

J A Oduma¹, E N Waindi, S Gombe, E O Wango

Affiliation

¹ Reproductive Biology Unit, University of Nairobi, Kenya.

PMID: 8013744
DOI: 10.1016/0020-711x(94)90017-5

Abstract

1. An androgen binding protein(s) has been partially purified from cell plasma membranes of dog epididymides. 2. The protein(s) has a pI of 5.3 and an association constant of (1.13 x 10(9) M-1). 3. Conclusive demonstration of androgen receptors in epididymal plasma membranes would be of significance in understanding epididymal physiology.

MeSH terms

Androgen-Binding Protein / isolation & purification*
Androgen-Binding Protein / metabolism
Animals
Cell Membrane / chemistry
Cell Membrane / metabolism
Dogs
Electrophoresis, Polyacrylamide Gel
Epididymis / chemistry*
Epididymis / metabolism
Isoelectric Point
Kinetics
Male
Molecular Weight
Receptors, Androgen / isolation & purification
Receptors, Androgen / metabolism

Substances

Androgen-Binding Protein
Receptors, Androgen