Abstract
Poly(methacrylic acid) hydrogels were tested for oral delivery of a vaccine against Pasteurella haemolytica infection in cattle. Culture supernatants of P haemolytica, the most common bacterium associated with pneumonia in cattle, were used as the antigens in the vaccine. Hydrogels containing culture supernatants were administered orally to calves. Calves were then challenge-exposed with virulent P haemolytica. Calves were euthanatized 3 days after challenge exposure. The lungs of each calf were scored for severity and size of pneumonic lesions. Results indicated that vaccinated calves had smaller, less severe pneumonic lesions and lived longer than nonvaccinated calves. These results indicated that hydrogels can be used to deliver vaccines orally to calves to enhance resistance to pneumonia caused by P haemolytica.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Oral
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Animals
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Antibodies, Bacterial / biosynthesis
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Antibodies, Bacterial / immunology
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Antigens, Bacterial / immunology
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Bacterial Vaccines / administration & dosage*
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Bacterial Vaccines / immunology
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Cattle
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Cattle Diseases / immunology
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Cattle Diseases / microbiology
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Cattle Diseases / prevention & control*
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Culture Media, Conditioned
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Enzyme-Linked Immunosorbent Assay
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Immunoblotting
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Mannheimia haemolytica / immunology*
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Pasteurella Infections / microbiology
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Pasteurella Infections / prevention & control
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Pasteurella Infections / veterinary*
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Polyethylene Glycols
Substances
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Antibodies, Bacterial
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Antigens, Bacterial
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Bacterial Vaccines
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Culture Media, Conditioned
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Polyethylene Glycols