Action of alpha-momorcharin, a ribosome inactivating protein, on cultured tumor cell lines

Gen Pharmacol. 1994 Jan;25(1):75-7. doi: 10.1016/0306-3623(94)90012-4.

Abstract

1. alpha-Momorcharin, a glycoprotein isolated from seeds of the bitter gourd, Momordica charantia inhibited incorporation of [3H]thymidine, [3H]leucine and [3H]uridine into P388 (mouse monocyte-macrophage), J774 (Balb/c macrophage), JAR (human placental choriocarcinoma) and sarcoma S180 cell lines. 2. The most potent inhibitory effect was exerted on P388 cells and the smallest effect on sarcoma cells. 3. alpha-Momorcharin also enhanced the tumoricidal effect of mouse macrophages on mouse mastocytomal (P815) cells.

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Drug Screening Assays, Antitumor
  • Humans
  • Leucine / metabolism
  • Leukemia P388 / drug therapy
  • Leukemia P388 / metabolism
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Mast-Cell Sarcoma / drug therapy
  • Mast-Cell Sarcoma / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Monocytes / drug effects
  • Monocytes / metabolism
  • Plant Extracts / pharmacology
  • Plant Proteins / pharmacology*
  • Ribosomal Proteins*
  • Ribosome Inactivating Proteins
  • Sarcoma 180 / drug therapy
  • Sarcoma 180 / metabolism
  • Thymidine / metabolism
  • Tritium
  • Tumor Cells, Cultured / drug effects
  • Uridine / metabolism

Substances

  • Antineoplastic Agents, Phytogenic
  • Plant Extracts
  • Plant Proteins
  • Ribosomal Proteins
  • Tritium
  • MMC protein, Momordica charantia
  • Ribosome Inactivating Proteins
  • Leucine
  • Thymidine
  • Uridine