Enhancement of expression of human granulocyte-macrophage colony stimulating factor by argU gene product in Escherichia coli

Z Hua; H Wang; D Chen; Y Chen; D Zhu

Enhancement of expression of human granulocyte-macrophage colony stimulating factor by argU gene product in Escherichia coli

Biochem Mol Biol Int. 1994 Mar;32(3):537-43.

Authors

Z Hua¹, H Wang, D Chen, Y Chen, D Zhu

Affiliation

¹ Department of Biochemistry, Nanjing University, P. R. China.

PMID: 8032321

Abstract

Human granulocyte-macrophage colony stimulating factor (hGM-CSF) was cloned into expression vector pIN III-ompA1 and expressed in Escherichia coli JA221. When supplementation with a minor tRNA(AGA/AGG)Arg encoded by the E. coli argU gene, the expression level of hGM-CSF was raised about 3-4-fold, although there is only one rare AGG codon in hGM-CSF cDNA gene.

MeSH terms

Base Sequence
Blotting, Western
Bone Marrow Cells
Cells, Cultured
Colony-Forming Units Assay
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Escherichia coli / genetics
Escherichia coli / metabolism*
Gene Expression
Genes, Bacterial*
Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis*
Granulocyte-Macrophage Colony-Stimulating Factor / isolation & purification
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
Hematopoietic Stem Cells / cytology
Hematopoietic Stem Cells / drug effects
Humans
Molecular Sequence Data
Molecular Weight
Plasmids
RNA, Transfer, Arg / genetics
RNA, Transfer, Arg / metabolism*
Recombinant Proteins / biosynthesis*
Recombinant Proteins / isolation & purification
Recombinant Proteins / pharmacology
Restriction Mapping

Substances

RNA, Transfer, Arg
Recombinant Proteins
Granulocyte-Macrophage Colony-Stimulating Factor