9 month human fetal gastric epithelial cells were primarily cultured and infected with SV40 virus. About 3 weeks after infection the transformed-like cells showed up and were isolated when most of the normal cells senesced. One transformed clone named GES-1 were analyzed biologically. The results indicated integration of the SV40 T gene and its expression in the cell nuclei and the cells became hyperploid in chromosomes. This cells also maintained a normal cytoskeleton, positive in PAS reaction and were non-tumorigenic in nude mice. The established cell line may be served as an important model system in study of human gastric carcinogenesis.