Abstract
Ruminal fluid which was enriched with trans-aconitate yielded a gram-negative diplococcus (strain AO) which was identified by 16S ribosomal DNA sequence analysis as Acidaminococcus fermentans. In contrast to the original description, the A. fermentans type strain and strain AO were found to utilize citrate as an energy source and to produce hydrogen and hydrogen sulfide. The descriptions of the genus and species are emended accordingly.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Aconitic Acid / metabolism*
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Animals
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Cattle
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Citrates / metabolism*
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Citric Acid
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DNA, Bacterial / genetics
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DNA, Ribosomal / genetics
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Gram-Negative Anaerobic Bacteria / classification*
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Gram-Negative Anaerobic Bacteria / genetics
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Gram-Negative Anaerobic Bacteria / metabolism*
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Molecular Sequence Data
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Oxidation-Reduction
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Rumen / microbiology
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Species Specificity
Substances
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Citrates
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DNA, Bacterial
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DNA, Ribosomal
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Citric Acid
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Aconitic Acid
Associated data
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GENBANK/X77951
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GENBANK/X78017