A long range restriction map spanning the myxoid liposarcoma breakpoint in the q13-14 region of human chromosome 12

A Forus; P F Kools; E F Schoenmakers; W J Van de Ven; O Myklebost

doi:10.1007/BF00208280

A long range restriction map spanning the myxoid liposarcoma breakpoint in the q13-14 region of human chromosome 12

Hum Genet. 1994 Sep;94(3):259-64. doi: 10.1007/BF00208280.

Authors

A Forus¹, P F Kools, E F Schoenmakers, W J Van de Ven, O Myklebost

Affiliation

¹ Department of Tumor Biology, Norwegian Radium Hospital, Oslo.

PMID: 8076941
DOI: 10.1007/BF00208280

Abstract

We have used pulsed-field gel electrophoresis to construct a long range restriction map of the myxoid liposarcoma (MLS) breakpoint region in 12q13-14. The CHOP/GADD153 gene, consistently translocated in myxoid liposarcomas, is located less than 55 kb from the putative oncogene GLI. We have used fluorescent in situ hybridization to orient the map with respect to the chromosome, and to show that GLI (and thus A2MR) is located proximal to the MLS breakpoint.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CCAAT-Enhancer-Binding Proteins*
Chromosome Aberrations*
Chromosome Banding
Chromosomes, Human, Pair 12*
DNA, Neoplasm / analysis
Electrophoresis, Gel, Pulsed-Field
Humans
In Situ Hybridization, Fluorescence
Liposarcoma, Myxoid / genetics*
Oncogenes
Proteins / genetics
Restriction Mapping*
Transcription Factor CHOP
Transcription Factors*
Tumor Cells, Cultured

Substances

CCAAT-Enhancer-Binding Proteins
DDIT3 protein, human
DNA, Neoplasm
Proteins
Transcription Factors
Transcription Factor CHOP