Effects of sterigmatocystin and T-2 toxin on the induction of unscheduled DNA synthesis in primary cultures of human gastric epithelial cells

Nat Toxins. 1994;2(3):115-9. doi: 10.1002/nt.2620020305.

Abstract

Primary cultures of human gastric epithelial cells were tested for induction of unscheduled DNA synthesis (UDS) by sterigmatocystin (ST) and T-2 toxin. Autoradiographic results indicated that ST (10(-6)-10(-4)M) induced UDS in the presence of S9 activation system. The repair rates were 24-91% (net grains > or = 3) and 2-71% (net grains > or = 5). T-2 toxin did not induce UDS in this study.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Biotransformation
  • Cell Division / drug effects
  • Cell Survival / drug effects
  • Cells, Cultured
  • DNA / biosynthesis*
  • Dose-Response Relationship, Drug
  • Epithelial Cells
  • Epithelium / drug effects
  • Gastric Mucosa / cytology
  • Gastric Mucosa / drug effects*
  • Gastric Mucosa / metabolism
  • Humans
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / metabolism
  • Rats
  • Sterigmatocystin / pharmacokinetics
  • Sterigmatocystin / toxicity*
  • T-2 Toxin / pharmacokinetics
  • T-2 Toxin / toxicity*

Substances

  • Sterigmatocystin
  • DNA
  • T-2 Toxin