Sequencing for 4 single-copy DNA segments from human chromosome 8q24.1

J H Xia; Q G Ruan; X X He; L L Li; L Huang; H X Deng; L Y Li

Sequencing for 4 single-copy DNA segments from human chromosome 8q24.1

Chin Med J (Engl). 1994 Apr;107(4):257-9.

Authors

J H Xia¹, Q G Ruan, X X He, L L Li, L Huang, H X Deng, L Y Li

Affiliation

¹ State Key Laboratory of Medical Genetics, Human Medical University, Changsha.

PMID: 8088191

Abstract

A successful method for constructing human chromosome 8q24.1 band specific probe pool by microdissection and microcloning is described in this paper. The probe pool was proved to be band-specific by fluorescence in situ hybridization. Through Southern blot analysis, 34 single copy DNA segments were obtained and four of them were sequenced. It is possible to provide useful landmarks for constructing human chromosome 8q24.1 physical map and STSs map.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Blotting, Southern
Chromosomes, Human, Pair 8*
DNA / genetics
Humans
In Situ Hybridization
Molecular Sequence Data
Sequence Analysis, DNA*

Substances

DNA

Associated data

GENBANK/S72100
GENBANK/S72119
GENBANK/S72123
GENBANK/S72124