Physical mapping of inhibin beta-A in domestic cattle

Mamm Genome. 1993;4(6):328-32. doi: 10.1007/BF00357092.

Abstract

The gene for the beta-A subunit of inhibin (INHBA) was assigned to bovine syntenic group U13 by bovine x rodent hybrid somatic cells and the polymerase chain reaction (PCR). A 482-bp PCR fragment was used to clone a 37-kb cosmid. This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). This is the first assignment of a U13 marker to a bovine chromosome. A restriction fragment length polymorphism (RFLP) was detected with PstI within the INHBA cosmid.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • CHO Cells
  • Cattle / genetics*
  • Chromosome Mapping*
  • Cricetinae
  • DNA, Single-Stranded
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence
  • Inhibins / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Genetic
  • Polymorphism, Restriction Fragment Length

Substances

  • DNA, Single-Stranded
  • Inhibins