Abstract
Treatment of Caco-2 cells with forskolin (25 microM) or monensin (1 microM) has previously been shown to cause a marked decrease in the level of sucrase-isomaltase (SI) mRNA, without any effect on the expression of dipeptidylpeptidase IV (DPP-IV). In the present work, we report that there is no significant difference in the stability of SI mRNA between control and treated cells. On the other hand, we demonstrate a decrease in the transcription rate of SI mRNA which is sufficient to account for the decrease in the steady-state level of SI mRNA both in forskolin- and monensin-treated Caco-2 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blotting, Northern
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Colforsin / pharmacology*
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DNA Probes
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Dipeptidyl Peptidase 4
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Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / metabolism
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Electrophoresis, Agar Gel
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Gene Expression Regulation, Enzymologic / drug effects*
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Humans
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Monensin / pharmacology*
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Oligo-1,6-Glucosidase / genetics
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Oligo-1,6-Glucosidase / metabolism*
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RNA, Messenger / metabolism*
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Sucrase / genetics
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Sucrase / metabolism*
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Transcription, Genetic / drug effects*
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Tumor Cells, Cultured
Substances
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DNA Probes
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RNA, Messenger
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Colforsin
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Monensin
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Oligo-1,6-Glucosidase
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Sucrase
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Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
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Dipeptidyl Peptidase 4