Identification of feline- and canine-like rotaviruses isolated from humans by restriction fragment length polymorphism assay

J Clin Microbiol. 1993 Jul;31(7):1783-7. doi: 10.1128/jcm.31.7.1783-1787.1993.

Abstract

Restriction fragment length polymorphism assay of reverse-transcribed and polymerase chain reaction-amplified rotavirus gene segment 9 was developed to differentiate human serotype 3 rotaviruses from animal serotype 3 rotaviruses. On the basis of similarities or differences in HinfI and DdeI restriction profiles, unusual group A serotype 3 human rotaviruses that belonged to subgroup I were shown to be of feline and canine origin. By this approach, the new human rotavirus isolates 5193, AU-387, AU-720, AU-785 and AU-1115 were shown to resemble certain feline-like human rotaviruses. Similar results were previously obtained by Nakagomi et al. (O. Nakagomi, A. Hoshima, Y. Aboudy, I. Shif, M. Mochizuki, T. Nakagomi, and T. Gotlieb-Stematsky. J. Clin. Microbiol. 28:1198-1203, 1990) by using RNA-RNA cross hybridization with established feline rotaviruses. The restriction fragment length polymorphism assay can provide fast and valuable information on the interspecies transmission of rotaviruses in nature.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cats / microbiology
  • Dogs / microbiology
  • Gastroenteritis / microbiology
  • Genome, Viral
  • Humans
  • Polymorphism, Restriction Fragment Length*
  • Rotavirus / classification
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification*
  • Rotavirus Infections / microbiology
  • Rotavirus Infections / transmission
  • Serotyping
  • Species Specificity