4',6-Diamidino 2-phenylindole is a new reversible inhibitor of diamine oxidase and S-adenosyl-L-methionine decarboxylase from mammalian tissues

C Cubría; M Alvarez-Bujidos; A Negro; R Balaña-Fouce; D Ordóñez

doi:10.1016/0742-8413(93)90203-w

4',6-Diamidino 2-phenylindole is a new reversible inhibitor of diamine oxidase and S-adenosyl-L-methionine decarboxylase from mammalian tissues

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1993 Jun;105(2):251-4. doi: 10.1016/0742-8413(93)90203-w.

Authors

C Cubría¹, M Alvarez-Bujidos, A Negro, R Balaña-Fouce, D Ordóñez

Affiliation

¹ Departamento de Fisiología, Farmacología y Toxicología, Universidad de León, Spain.

PMID: 8103731
DOI: 10.1016/0742-8413(93)90203-w

Abstract

1. 4',6-Diamidino-2-phenylindole is a powerful reversible inhibitor of porcine kidney diamine oxidase and partially purified rabbit liver S-adenosyl-L-methionine decarboxylase. 2. This diamidine has shown to be a competitive inhibitor of porcine kidney diamine oxidase with a Ki value of 13 microM. 3. A similar inhibitory pattern has been found on rat liver S-adenosyl-L-methionine decarboxylase with an estimated Ki of 21 microM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosylmethionine Decarboxylase / antagonists & inhibitors*
Amine Oxidase (Copper-Containing) / antagonists & inhibitors*
Animals
Indoles / pharmacology*
Intercalating Agents / pharmacology*
Kinetics
Liver / drug effects
Liver / enzymology
Molecular Structure
Rabbits / metabolism*
Swine

Substances

Indoles
Intercalating Agents
DAPI
Amine Oxidase (Copper-Containing)
Adenosylmethionine Decarboxylase