The corn inhibitor of blood coagulation factor XIIa. Crystallization and preliminary crystallographic analysis

J Mol Biol. 1994 Feb 11;236(1):385-7. doi: 10.1006/jmbi.1994.1147.

Abstract

A 13.6 kDa protein from corn seeds is known to be a highly selective inhibitor of human blood coagulation Factor XIIa (or activated Hageman factor). We have crystallized this inhibitor at 23 degrees C and pH 7.5 from a solution of 30% polyethylene glycol 400, 0.2 M MgCl2, and 0.1 M Hepes. The crystals diffract to at least 2.1 A resolution. The space group is P4(2)2(1)2 with a = b = 57.15 A and c = 80.5 A. The crystals contain 51% solvent. Two heavy atom derivatives have been identified.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cloning, Molecular
  • Crystallization
  • Crystallography, X-Ray / methods
  • Factor XIIa / antagonists & inhibitors*
  • Humans
  • Hydrogen-Ion Concentration
  • Molecular Weight
  • Recombinant Proteins / chemistry
  • Zea mays*

Substances

  • Recombinant Proteins
  • Factor XIIa