Molecular analysis of a patient with hydrops fetalis caused by beta-glucuronidase deficiency, and evidence for additional pseudogenes

Hum Mutat. 1993;2(6):443-5. doi: 10.1002/humu.1380020604.

Abstract

A patient with hydrops fetalis caused by beta-glucuronidase deficiency was found to be homozygous for a C to T transition at nucleotide position 672 in his cDNA. Genomic analysis showed the presence of pseudogenes for the beta-glucuronidase gene. After separation of PCR products of the gene and the pseudogenes it was shown that the patient and his father were heterozygous for the C-T 672 transition and the mother did not carry the mutation.

MeSH terms

  • Base Sequence
  • DNA
  • Glucuronidase / deficiency*
  • Heterozygote
  • Homozygote
  • Humans
  • Hydrops Fetalis / enzymology
  • Hydrops Fetalis / genetics*
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Pseudogenes*
  • Sequence Homology, Nucleic Acid

Substances

  • DNA
  • Glucuronidase