Serum bactericidal activity (SBA) was determined against ten strains each of Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris and Enterobacter cloacae in six volunteers 1 h and 4 h after intravenous infusion of 1 g and 2 g cefotaxime and cefmenoxime, and 2 g flomoxef, and against ten strains of Pseudomonas aeruginosa after infusion of 1 g and 2 g ceftazidime. Flomoxef showed the highest SBA against methicillin-susceptible Staphylococcus aureus. All cephalosporins had high SBA against gram-negative rods. Cefotaxime had the highest SBA against Klebsiella pneumoniae and Enterobacter cloacae. The SBA against Pseudomonas aeruginosa after 1 g and 2 g doses of ceftazidime was very similar.