Insulin-like growth factors counteract the effect of interleukin 1 beta on type II phospholipase A2 expression and arachidonic acid release by rabbit articular chondrocytes

FEBS Lett. 1994 Feb 28;340(1-2):51-5. doi: 10.1016/0014-5793(94)80171-1.

Abstract

Interleukin 1 beta was found to stimulate arachidonic acid release, and the synthesis and secretion of type II phospholipase A2 by rabbit articular chondrocytes in vitro. Interleukin 1 beta had no effect on the level of cytosolic phospholipase A2 mRNA. Insulin-like growth factors, which help stabilize the cartilage matrix, reduced the effect of interleukin 1 beta on type II phospholipase A2 activity and mRNA level, and decreased the Interleukin 1 beta-stimulated arachidonic acid release to the basal values. This suggests that type II phospholipase A2 plays a key role in arachidonic acid release from rabbit articular chondrocytes and that insulin-like growth factors counteract the effect of interleukin 1 beta. They may therefore be considered as potential antiinflammatory agents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arachidonic Acid / metabolism*
  • Cartilage, Articular / cytology
  • Cartilage, Articular / drug effects*
  • Cartilage, Articular / enzymology
  • Cartilage, Articular / metabolism
  • In Vitro Techniques
  • Insulin-Like Growth Factor I / pharmacology*
  • Insulin-Like Growth Factor II / pharmacology*
  • Interleukin-1 / antagonists & inhibitors*
  • Interleukin-1 / pharmacology
  • Phospholipases A / biosynthesis*
  • Phospholipases A / genetics
  • Phospholipases A2
  • RNA, Messenger / metabolism
  • Rabbits

Substances

  • Interleukin-1
  • RNA, Messenger
  • Arachidonic Acid
  • Insulin-Like Growth Factor I
  • Insulin-Like Growth Factor II
  • Phospholipases A
  • Phospholipases A2